Demineralized dentin matrix combined with recombinant human bone morphogenetic protein-2 in rabbit calvarial defects
엄인웅, 황석현, 김영균, 김문영, 전상호, 류재준, 장현석,
소속 상세정보
엄인웅 ( Um In-Woong ) - Korea Tooth Bank
황석현 ( Hwang Suk-Hyun ) - Korea University Graduate School Department of Medicine
김영균 ( Kim Young-Kyun ) - Seoul National University Bundang Hospital Section of Dentistry Department of Oral and Maxillofacial Surgery
김문영 ( Kim Moon-Young ) - Dankook University College of Dentistry Department of Oral and Maxillofacial Surgery
전상호 ( Jun Sang-Ho ) - Korea University Anam Hospital Department of Dentistry
류재준 ( Ryu Jae-Jun ) - Korea University Anam Hospital Department of Dentistry
장현석 ( Jang Hyon-Seok ) - Korea University Ansan Hospital Department of Dentistry
KMID : 0355620160420020090
Abstract
Objectives: The aim of this study was to compare the osteogenic effects of demineralized dentin matrix (DDM) combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) in rabbit calvarial defects with DDM and anorganic bovine bone (ABB) combined with rhBMP-2.
Materials and Methods: Four round defects with 8-mm diameters were created in each rabbit calvaria. Each defect was treated with one of the following: 1) DDM, 2) ABB/rhBMP-2, or 3) DDM/rhBMP-2. The rhBMP-2 was combined with DDM and ABB according to a stepwise dry and dip lyophilizing protocol. Histological and microcomputed tomography (?CT) analyses were performed to measure the amount of bone formation and bone volume after 2- and 8-week healing intervals.
Results: Upon histological observation at two weeks, the DDM and ABB/rhBMP-2 groups showed osteoconductive bone formation, while the DDM/rhBMP-2 group showed osteoconductive and osteoinductive bone formation. New bone formation was higher in DDM/rhBMP-2, DDM and ABB decreasing order. The amounts of bone formation were very similar at two weeks; however, at eight weeks, the DDM/rhBMP-2 group showed a two-fold greater amount of bone formation compared to the DDM and ABB/rhBMP-2 groups. The ?CT analysis showed markedly increased bone volume in the DDM/rhBMP-2 group at eight weeks compared with that of the DDM group. Notably, there was a slight decrease in bone volume in the ABB/rhBMP-2 group at eight weeks. There were no significant differences among the DDM, ABB/rhBMP-2, and DDM/rhBMP-2 groups at two or eight weeks.
Conclusion: Within the limitations of this study, DDM appears to be a suitable carrier for rhBMP-2 in orthotopic sites.
키워드
Demineralized dentin matrix; Recombinant human bone morphogenetic protein-2; Microcomputed tomography; Histomorphometric analysis
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